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differentiation 31 cd31 affinity purified polyclonal ab  (R&D Systems)


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    Structured Review

    R&D Systems differentiation 31 cd31 affinity purified polyclonal ab
    A) 2D cross sectional US image in a representative MIA PaCa-2 tumor. B) H&E stain of the same tumor cross section shown in A,C. C) 2D cross sectional image of HbT overlayed on US in a representative MIA PaCa-2 tumor with white arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD. D) IF stain of the same tumor cross section shown in A,C with blue representing DAPI and red representing <t>CD31.</t> White arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD according to the HbT image. E) Plot of normalized average CD31 intensity in a 1 mm x 1 mm ROI versus the fraction of pixels in the ROI labelled as HVD. All scale bars shown represent 2 mm.
    Differentiation 31 Cd31 Affinity Purified Polyclonal Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd31+pecam+1+affinity+purified+polyclonal+ab/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pmc11840746-130-19-27
    Average 98 stars, based on 1263 article reviews
    differentiation 31 cd31 affinity purified polyclonal ab - by Bioz Stars, 2026-09
    98/100 stars

    Images

    1) Product Images from "Vascular regional analysis unveils differential responses to anti-angiogenic therapy in pancreatic xenografts through macroscopic photoacoustic imaging"

    Article Title: Vascular regional analysis unveils differential responses to anti-angiogenic therapy in pancreatic xenografts through macroscopic photoacoustic imaging

    Journal: Theranostics

    doi: 10.7150/thno.99361

    A) 2D cross sectional US image in a representative MIA PaCa-2 tumor. B) H&E stain of the same tumor cross section shown in A,C. C) 2D cross sectional image of HbT overlayed on US in a representative MIA PaCa-2 tumor with white arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD. D) IF stain of the same tumor cross section shown in A,C with blue representing DAPI and red representing CD31. White arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD according to the HbT image. E) Plot of normalized average CD31 intensity in a 1 mm x 1 mm ROI versus the fraction of pixels in the ROI labelled as HVD. All scale bars shown represent 2 mm.
    Figure Legend Snippet: A) 2D cross sectional US image in a representative MIA PaCa-2 tumor. B) H&E stain of the same tumor cross section shown in A,C. C) 2D cross sectional image of HbT overlayed on US in a representative MIA PaCa-2 tumor with white arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD. D) IF stain of the same tumor cross section shown in A,C with blue representing DAPI and red representing CD31. White arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD according to the HbT image. E) Plot of normalized average CD31 intensity in a 1 mm x 1 mm ROI versus the fraction of pixels in the ROI labelled as HVD. All scale bars shown represent 2 mm.

    Techniques Used: Staining

    A-B) Representative AsPC-1 tumors stained for CD31 (red) and αSMA (green) overlaid on DAPI (blue) for the control (A) and sunitinib (B) treated group. C) Bar graph comparing αSMA + / CD31 + cell ratio between the control (black) and sunitinib (red) groups. D-E) Representative MIA PaCa-2 tumors stained with CD31 (red) and αSMA (green) overlaid on DAPI (blue) for the control (D) and sunitinib (E) treated group. F) Bar graph (Mean +/- SEM) comparing αSMA + / CD31 + cell ratio between the control (black) and sunitinib (red) groups for MIA PaCa-2. G-H) Scatter plot of αSMA + / CD31 + versus the HVD ∆StO₂ between D(8) and D(-1) (G) HVD fraction on D(8) (H) for each histological sample with points corresponding to MIA PaCa-2 tumors outlined in green, and AsPC-1 tumors outlined in blue. I-J) Representative AsPC-1 tumors stained for CD31 (red) and TL (green) overlaid on DAPI (blue) for the control (I) and sunitinib (J) treated group. K) Bar graph comparing TL + / CD31 + cell ratio between the control (black) and sunitinib (red) groups. L -M) Representative MIA PaCa-2 tumors stained with CD31 (red) and TL (green) overlaid on DAPI (blue) for the control (L) and sunitinib (M) treated group. N) Bar graph comparing TL + / CD31 + cell ratio between the control (black) and sunitinib (red) groups for MIA PaCa-2. O-P) Scatter plot of ΤL + / CD31 + versus the HVD ∆StO₂ between D(8) and D(-1) (O) HVD fraction on D(8) (P) for each histological sample with points corresponding to MIA PaCa-2 tumors outlined in green, and AsPC-1 tumors outlined in blue. It is to be noted that all four stains DAPI, TL, αSMA and CD31 are performed on the same section. For display purposes we showed DAPI, TL, CD 31 and DAPI, αSMA and CD31 images separately. All scale bars = 125 μm, All error bars shown represent SEM. p-values: * < 0.05, ** < 0.01, *** < 0.001
    Figure Legend Snippet: A-B) Representative AsPC-1 tumors stained for CD31 (red) and αSMA (green) overlaid on DAPI (blue) for the control (A) and sunitinib (B) treated group. C) Bar graph comparing αSMA + / CD31 + cell ratio between the control (black) and sunitinib (red) groups. D-E) Representative MIA PaCa-2 tumors stained with CD31 (red) and αSMA (green) overlaid on DAPI (blue) for the control (D) and sunitinib (E) treated group. F) Bar graph (Mean +/- SEM) comparing αSMA + / CD31 + cell ratio between the control (black) and sunitinib (red) groups for MIA PaCa-2. G-H) Scatter plot of αSMA + / CD31 + versus the HVD ∆StO₂ between D(8) and D(-1) (G) HVD fraction on D(8) (H) for each histological sample with points corresponding to MIA PaCa-2 tumors outlined in green, and AsPC-1 tumors outlined in blue. I-J) Representative AsPC-1 tumors stained for CD31 (red) and TL (green) overlaid on DAPI (blue) for the control (I) and sunitinib (J) treated group. K) Bar graph comparing TL + / CD31 + cell ratio between the control (black) and sunitinib (red) groups. L -M) Representative MIA PaCa-2 tumors stained with CD31 (red) and TL (green) overlaid on DAPI (blue) for the control (L) and sunitinib (M) treated group. N) Bar graph comparing TL + / CD31 + cell ratio between the control (black) and sunitinib (red) groups for MIA PaCa-2. O-P) Scatter plot of ΤL + / CD31 + versus the HVD ∆StO₂ between D(8) and D(-1) (O) HVD fraction on D(8) (P) for each histological sample with points corresponding to MIA PaCa-2 tumors outlined in green, and AsPC-1 tumors outlined in blue. It is to be noted that all four stains DAPI, TL, αSMA and CD31 are performed on the same section. For display purposes we showed DAPI, TL, CD 31 and DAPI, αSMA and CD31 images separately. All scale bars = 125 μm, All error bars shown represent SEM. p-values: * < 0.05, ** < 0.01, *** < 0.001

    Techniques Used: Staining, Control

    Related Articles

    Affinity Purification:

    Article Title: Prediction of tumor recurrence and therapy monitoring using ultrasound-guided photoacoustic imaging.
    Article Snippet: A blocking solution (Dako Protein Block Reagent) was applied for 1-hr at room temperature, followed by application of the antibody at ∼10 μg/mL overnight at 4 °C. .. Mouse CD31/PECAM-1 Affinity Purified Polyclonal Ab (R&D Systems Inc) and HP-Red549 (Hypoxyprobe Inc) were used for staining the microvasculature and hypoxic areas in the tumor section. .. The slides were washed in PBS and secondary antibody (Donkey Anti-Goat IgG NL493 Affinity Purified PAb (R&D systems Inc) was applied for 2-hrs at room temperature.

    Article Title: Vascular regional analysis unveils differential responses to anti-angiogenic therapy in pancreatic xenografts through macroscopic photoacoustic imaging
    Article Snippet: The tissue sections were then blocked with a 1x concentration blocking solution (BlockerTM BSA; #37525, ThermoFisher ScientificTM) for 1 hour at room temperature. .. The immunostaining of vasculature within the tumor sections was performed using a primary antibody, specifically the Mouse CD31/PECAM-1 Affinity Purified Polyclonal Ab (#AF3628, R&D Systems Inc). ..

    Article Title: Ultrasound-guided Photoacoustic image Annotation Toolkit in MATLAB (PHANTOM) for preclinical applications
    Article Snippet: Following this, a blocking solution with a 1x concentration (BSA, Thermo ScientificTM) was applied to the sections, allowing incubation period of 1 hour at room temperature. .. For the immunostaining of microvasculature and hypoxic regions within the tumor sections, primary antibodies, namely Mouse CD31/PECAM-1 Affinity Purified Polyclonal Ab (R&D Systems Inc) and FITC-Mab (Hydroxyprobe Inc), were incubated with the tissue sections at an approximate concentration of 10 μg/mL. ..

    Article Title: Ultrasound-guided photoacoustic image annotation toolkit in MATLAB (PHANTOM) for preclinical applications
    Article Snippet: Following this, a blocking solution with a 1x concentration (BSA, Thermo ScientificTM) was applied to the sections, allowing incubation period of 1 hour at room temperature. .. For the immunostaining of microvasculature and hypoxic regions within the tumor sections, primary antibodies, namely Mouse CD31/PECAM-1 Affinity Purified Polyclonal Ab (R&D Systems Inc) and FITC-Mab (Hydroxyprobe Inc), were incubated with the tissue sections at an approximate concentration of 10 μg/mL. ..

    Article Title: Impact of ectopic calcification on vascular oxygenation in placental dysfunction models: Simultaneous in vivo assessment with multi-parametric ultrasound and photoacoustic imaging
    Article Snippet: Following this, a blocking solution with a 1x concentration (Thermo Scientific) was applied to the sections for an incubation period of 1 hour at room temperature. .. Primary antibodies, namely mouse CD31/PECAM-1 affinity purified polyclonal Ab (R&D Systems Inc) and FITC-Mab (Hydroxyprobe Inc), were administered at an approximate concentration of 10 μg/mL. ..

    Staining:

    Article Title: Prediction of tumor recurrence and therapy monitoring using ultrasound-guided photoacoustic imaging.
    Article Snippet: A blocking solution (Dako Protein Block Reagent) was applied for 1-hr at room temperature, followed by application of the antibody at ∼10 μg/mL overnight at 4 °C. .. Mouse CD31/PECAM-1 Affinity Purified Polyclonal Ab (R&D Systems Inc) and HP-Red549 (Hypoxyprobe Inc) were used for staining the microvasculature and hypoxic areas in the tumor section. .. The slides were washed in PBS and secondary antibody (Donkey Anti-Goat IgG NL493 Affinity Purified PAb (R&D systems Inc) was applied for 2-hrs at room temperature.

    Immunostaining:

    Article Title: Vascular regional analysis unveils differential responses to anti-angiogenic therapy in pancreatic xenografts through macroscopic photoacoustic imaging
    Article Snippet: The tissue sections were then blocked with a 1x concentration blocking solution (BlockerTM BSA; #37525, ThermoFisher ScientificTM) for 1 hour at room temperature. .. The immunostaining of vasculature within the tumor sections was performed using a primary antibody, specifically the Mouse CD31/PECAM-1 Affinity Purified Polyclonal Ab (#AF3628, R&D Systems Inc). ..

    Article Title: Ultrasound-guided Photoacoustic image Annotation Toolkit in MATLAB (PHANTOM) for preclinical applications
    Article Snippet: Following this, a blocking solution with a 1x concentration (BSA, Thermo ScientificTM) was applied to the sections, allowing incubation period of 1 hour at room temperature. .. For the immunostaining of microvasculature and hypoxic regions within the tumor sections, primary antibodies, namely Mouse CD31/PECAM-1 Affinity Purified Polyclonal Ab (R&D Systems Inc) and FITC-Mab (Hydroxyprobe Inc), were incubated with the tissue sections at an approximate concentration of 10 μg/mL. ..

    Article Title: Ultrasound-guided photoacoustic image annotation toolkit in MATLAB (PHANTOM) for preclinical applications
    Article Snippet: Following this, a blocking solution with a 1x concentration (BSA, Thermo ScientificTM) was applied to the sections, allowing incubation period of 1 hour at room temperature. .. For the immunostaining of microvasculature and hypoxic regions within the tumor sections, primary antibodies, namely Mouse CD31/PECAM-1 Affinity Purified Polyclonal Ab (R&D Systems Inc) and FITC-Mab (Hydroxyprobe Inc), were incubated with the tissue sections at an approximate concentration of 10 μg/mL. ..

    Incubation:

    Article Title: Ultrasound-guided Photoacoustic image Annotation Toolkit in MATLAB (PHANTOM) for preclinical applications
    Article Snippet: Following this, a blocking solution with a 1x concentration (BSA, Thermo ScientificTM) was applied to the sections, allowing incubation period of 1 hour at room temperature. .. For the immunostaining of microvasculature and hypoxic regions within the tumor sections, primary antibodies, namely Mouse CD31/PECAM-1 Affinity Purified Polyclonal Ab (R&D Systems Inc) and FITC-Mab (Hydroxyprobe Inc), were incubated with the tissue sections at an approximate concentration of 10 μg/mL. ..

    Article Title: Ultrasound-guided photoacoustic image annotation toolkit in MATLAB (PHANTOM) for preclinical applications
    Article Snippet: Following this, a blocking solution with a 1x concentration (BSA, Thermo ScientificTM) was applied to the sections, allowing incubation period of 1 hour at room temperature. .. For the immunostaining of microvasculature and hypoxic regions within the tumor sections, primary antibodies, namely Mouse CD31/PECAM-1 Affinity Purified Polyclonal Ab (R&D Systems Inc) and FITC-Mab (Hydroxyprobe Inc), were incubated with the tissue sections at an approximate concentration of 10 μg/mL. ..

    Concentration Assay:

    Article Title: Ultrasound-guided Photoacoustic image Annotation Toolkit in MATLAB (PHANTOM) for preclinical applications
    Article Snippet: Following this, a blocking solution with a 1x concentration (BSA, Thermo ScientificTM) was applied to the sections, allowing incubation period of 1 hour at room temperature. .. For the immunostaining of microvasculature and hypoxic regions within the tumor sections, primary antibodies, namely Mouse CD31/PECAM-1 Affinity Purified Polyclonal Ab (R&D Systems Inc) and FITC-Mab (Hydroxyprobe Inc), were incubated with the tissue sections at an approximate concentration of 10 μg/mL. ..

    Article Title: Ultrasound-guided photoacoustic image annotation toolkit in MATLAB (PHANTOM) for preclinical applications
    Article Snippet: Following this, a blocking solution with a 1x concentration (BSA, Thermo ScientificTM) was applied to the sections, allowing incubation period of 1 hour at room temperature. .. For the immunostaining of microvasculature and hypoxic regions within the tumor sections, primary antibodies, namely Mouse CD31/PECAM-1 Affinity Purified Polyclonal Ab (R&D Systems Inc) and FITC-Mab (Hydroxyprobe Inc), were incubated with the tissue sections at an approximate concentration of 10 μg/mL. ..

    Article Title: Impact of ectopic calcification on vascular oxygenation in placental dysfunction models: Simultaneous in vivo assessment with multi-parametric ultrasound and photoacoustic imaging
    Article Snippet: Following this, a blocking solution with a 1x concentration (Thermo Scientific) was applied to the sections for an incubation period of 1 hour at room temperature. .. Primary antibodies, namely mouse CD31/PECAM-1 affinity purified polyclonal Ab (R&D Systems Inc) and FITC-Mab (Hydroxyprobe Inc), were administered at an approximate concentration of 10 μg/mL. ..



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    R&D Systems differentiation 31 cd31 affinity purified polyclonal ab
    A) 2D cross sectional US image in a representative MIA PaCa-2 tumor. B) H&E stain of the same tumor cross section shown in A,C. C) 2D cross sectional image of HbT overlayed on US in a representative MIA PaCa-2 tumor with white arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD. D) IF stain of the same tumor cross section shown in A,C with blue representing DAPI and red representing <t>CD31.</t> White arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD according to the HbT image. E) Plot of normalized average CD31 intensity in a 1 mm x 1 mm ROI versus the fraction of pixels in the ROI labelled as HVD. All scale bars shown represent 2 mm.
    Differentiation 31 Cd31 Affinity Purified Polyclonal Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd31+pecam+1+affinity+purified+polyclonal+ab/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pmc11840746-130-19-27
    Average 98 stars, based on 1 article reviews
    differentiation 31 cd31 affinity purified polyclonal ab - by Bioz Stars, 2026-09
    98/100 stars
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    R&D Systems mouse cd31 pecam 1 affinity purified polyclonal ab
    A) 2D cross sectional US image in a representative MIA PaCa-2 tumor. B) H&E stain of the same tumor cross section shown in A,C. C) 2D cross sectional image of HbT overlayed on US in a representative MIA PaCa-2 tumor with white arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD. D) IF stain of the same tumor cross section shown in A,C with blue representing DAPI and red representing <t>CD31.</t> White arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD according to the HbT image. E) Plot of normalized average CD31 intensity in a 1 mm x 1 mm ROI versus the fraction of pixels in the ROI labelled as HVD. All scale bars shown represent 2 mm.
    Mouse Cd31 Pecam 1 Affinity Purified Polyclonal Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd31+pecam+1+affinity+purified+polyclonal+ab/Mouse+CD31%2FPECAM-1+Antibody/pmc11648259-224-15-21
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    R&D Systems c mouse cd31 pecam 1 affinity purified polyclonal ab
    A) 2D cross sectional US image in a representative MIA PaCa-2 tumor. B) H&E stain of the same tumor cross section shown in A,C. C) 2D cross sectional image of HbT overlayed on US in a representative MIA PaCa-2 tumor with white arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD. D) IF stain of the same tumor cross section shown in A,C with blue representing DAPI and red representing <t>CD31.</t> White arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD according to the HbT image. E) Plot of normalized average CD31 intensity in a 1 mm x 1 mm ROI versus the fraction of pixels in the ROI labelled as HVD. All scale bars shown represent 2 mm.
    C Mouse Cd31 Pecam 1 Affinity Purified Polyclonal Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd31+pecam+1+affinity+purified+polyclonal+ab/Mouse+CD31%2FPECAM-1+Antibody/us10426388-502-26-33
    Average 94 stars, based on 1 article reviews
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    Image Search Results


    A) 2D cross sectional US image in a representative MIA PaCa-2 tumor. B) H&E stain of the same tumor cross section shown in A,C. C) 2D cross sectional image of HbT overlayed on US in a representative MIA PaCa-2 tumor with white arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD. D) IF stain of the same tumor cross section shown in A,C with blue representing DAPI and red representing CD31. White arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD according to the HbT image. E) Plot of normalized average CD31 intensity in a 1 mm x 1 mm ROI versus the fraction of pixels in the ROI labelled as HVD. All scale bars shown represent 2 mm.

    Journal: Theranostics

    Article Title: Vascular regional analysis unveils differential responses to anti-angiogenic therapy in pancreatic xenografts through macroscopic photoacoustic imaging

    doi: 10.7150/thno.99361

    Figure Lengend Snippet: A) 2D cross sectional US image in a representative MIA PaCa-2 tumor. B) H&E stain of the same tumor cross section shown in A,C. C) 2D cross sectional image of HbT overlayed on US in a representative MIA PaCa-2 tumor with white arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD. D) IF stain of the same tumor cross section shown in A,C with blue representing DAPI and red representing CD31. White arrows pointing towards areas of HVD and yellow arrows pointing towards areas of LVD according to the HbT image. E) Plot of normalized average CD31 intensity in a 1 mm x 1 mm ROI versus the fraction of pixels in the ROI labelled as HVD. All scale bars shown represent 2 mm.

    Article Snippet: The immunostaining of vasculature within the tumor sections was performed using two primary antibodies, namely the Mouse PECAM-1/cluster of differentiation 31 (CD31) Affinity Purified Polyclonal Ab (#AF3628, R&D Systems Inc) and Rabbit ACTA2/alpha-Smooth Muscle Actin (aSMA) Polyclonal Ab (#50-556-90, Fisher Scientific).

    Techniques: Staining

    A-B) Representative AsPC-1 tumors stained for CD31 (red) and αSMA (green) overlaid on DAPI (blue) for the control (A) and sunitinib (B) treated group. C) Bar graph comparing αSMA + / CD31 + cell ratio between the control (black) and sunitinib (red) groups. D-E) Representative MIA PaCa-2 tumors stained with CD31 (red) and αSMA (green) overlaid on DAPI (blue) for the control (D) and sunitinib (E) treated group. F) Bar graph (Mean +/- SEM) comparing αSMA + / CD31 + cell ratio between the control (black) and sunitinib (red) groups for MIA PaCa-2. G-H) Scatter plot of αSMA + / CD31 + versus the HVD ∆StO₂ between D(8) and D(-1) (G) HVD fraction on D(8) (H) for each histological sample with points corresponding to MIA PaCa-2 tumors outlined in green, and AsPC-1 tumors outlined in blue. I-J) Representative AsPC-1 tumors stained for CD31 (red) and TL (green) overlaid on DAPI (blue) for the control (I) and sunitinib (J) treated group. K) Bar graph comparing TL + / CD31 + cell ratio between the control (black) and sunitinib (red) groups. L -M) Representative MIA PaCa-2 tumors stained with CD31 (red) and TL (green) overlaid on DAPI (blue) for the control (L) and sunitinib (M) treated group. N) Bar graph comparing TL + / CD31 + cell ratio between the control (black) and sunitinib (red) groups for MIA PaCa-2. O-P) Scatter plot of ΤL + / CD31 + versus the HVD ∆StO₂ between D(8) and D(-1) (O) HVD fraction on D(8) (P) for each histological sample with points corresponding to MIA PaCa-2 tumors outlined in green, and AsPC-1 tumors outlined in blue. It is to be noted that all four stains DAPI, TL, αSMA and CD31 are performed on the same section. For display purposes we showed DAPI, TL, CD 31 and DAPI, αSMA and CD31 images separately. All scale bars = 125 μm, All error bars shown represent SEM. p-values: * < 0.05, ** < 0.01, *** < 0.001

    Journal: Theranostics

    Article Title: Vascular regional analysis unveils differential responses to anti-angiogenic therapy in pancreatic xenografts through macroscopic photoacoustic imaging

    doi: 10.7150/thno.99361

    Figure Lengend Snippet: A-B) Representative AsPC-1 tumors stained for CD31 (red) and αSMA (green) overlaid on DAPI (blue) for the control (A) and sunitinib (B) treated group. C) Bar graph comparing αSMA + / CD31 + cell ratio between the control (black) and sunitinib (red) groups. D-E) Representative MIA PaCa-2 tumors stained with CD31 (red) and αSMA (green) overlaid on DAPI (blue) for the control (D) and sunitinib (E) treated group. F) Bar graph (Mean +/- SEM) comparing αSMA + / CD31 + cell ratio between the control (black) and sunitinib (red) groups for MIA PaCa-2. G-H) Scatter plot of αSMA + / CD31 + versus the HVD ∆StO₂ between D(8) and D(-1) (G) HVD fraction on D(8) (H) for each histological sample with points corresponding to MIA PaCa-2 tumors outlined in green, and AsPC-1 tumors outlined in blue. I-J) Representative AsPC-1 tumors stained for CD31 (red) and TL (green) overlaid on DAPI (blue) for the control (I) and sunitinib (J) treated group. K) Bar graph comparing TL + / CD31 + cell ratio between the control (black) and sunitinib (red) groups. L -M) Representative MIA PaCa-2 tumors stained with CD31 (red) and TL (green) overlaid on DAPI (blue) for the control (L) and sunitinib (M) treated group. N) Bar graph comparing TL + / CD31 + cell ratio between the control (black) and sunitinib (red) groups for MIA PaCa-2. O-P) Scatter plot of ΤL + / CD31 + versus the HVD ∆StO₂ between D(8) and D(-1) (O) HVD fraction on D(8) (P) for each histological sample with points corresponding to MIA PaCa-2 tumors outlined in green, and AsPC-1 tumors outlined in blue. It is to be noted that all four stains DAPI, TL, αSMA and CD31 are performed on the same section. For display purposes we showed DAPI, TL, CD 31 and DAPI, αSMA and CD31 images separately. All scale bars = 125 μm, All error bars shown represent SEM. p-values: * < 0.05, ** < 0.01, *** < 0.001

    Article Snippet: The immunostaining of vasculature within the tumor sections was performed using two primary antibodies, namely the Mouse PECAM-1/cluster of differentiation 31 (CD31) Affinity Purified Polyclonal Ab (#AF3628, R&D Systems Inc) and Rabbit ACTA2/alpha-Smooth Muscle Actin (aSMA) Polyclonal Ab (#50-556-90, Fisher Scientific).

    Techniques: Staining, Control